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| ชื่อเรื่อง |
Immunocytochemical methods and protocols/ edited by Constance Oliver and Maria | | ISBN |
978-158-82-9463-0
| | พิมพ์ลักษณ์ |
New York, NY : Humana Press, c2010 | | เลขเรียก |
IM7 Im31I | | ครั้งที่พิมพ์ |
3rd ed. | | ลักษณะทางกายภาพ |
xii, 416 p. : ill. ; 27 cm | | หมายเหตุ |
Previous ed.: 1999 | | หมายเหตุ |
Contents: Overview of antibodies for immunochemistry / ConstanceOliver and Maria Célia Jamur
-- Introduction to thepurification of antibodies / Ana Cristina Grodzki and ElsaBerenstein
-- Antibody purification : ammonium sulfatefractionation or gel filtration / Ana Cristina Grodzki andElsa Berenstein
-- Antibody purification : ion-exchangechromatography / Ana Cristina Grodzki and Elsa Berenstein
-- Antibody purification : affinity chromatography
--Protein A and Protein G Sepharose / Ana Cristina Grodzkiand Elsa Berenstein
-- Conjugation of fluorochromes toantibodies / Su-Yau Mao and J. Michael Mullins
--Biotinylation of antibodies / Su-Yau Mao
-- Cell fixativesfor immunostaining / Maria Célia Jamur and ConstanceOliver
-- Permeabilization of cell membranes / Maria CéliaJamur and Constance Oliver
-- Preparation of frozensections for analysis / Gary L. Bratthauer -- Processingof cytological specimens / Gary L. Bratthauer --Processing of tissue culture cells / Gary L. Bratthauer --Processing of tissue specimens / Gary L. Bratthauer --Heat induced antigen retrieval for immunohistochemicalreactions in routinely processed paraffin sections /Laszlo Krenacs, Tibor Krenacs, Eva Stelkovics, and MarkRaffeld -- Fluorochromes : properties and characteristics/ J. Michael Mullins -- Direct immunofluorescent labelingof cells / Maria Veronica Dávila Pástor -- Fluorescencelabeling of surface antigens of attached or suspendedtissue-culture cells / Mark C. Willingham -- Fluorescencelabeling of intracellular antigens of attached orsuspended tissue-culture cells / Mark C. Willingham --Fluorescent visualization of macromolecules in Drosophilawhole mounts / Ricardo Guelerman Pinheiro Ramos, LucianaClaudia Herculano Machado, and Livia Maria Rosatto Moda --Overview of conventional fluorescence photomicrography /J. Michael Mullins -- Overview of confocal microscopy /William D. Swaim -- Overview of laser microbeamapplications as related to antibody targeting / P. ScottPine -- Immuno-laser capture microdissection of rat brainneurons for real time quantitative PCR / Denis G. Baskinand L. Scot Bastian -- Overview of antigen detectionthrough enzymatic activity / Gary L. Bratthauer -- Theperoxidase-antiperoxidase (PAP) method and other all-immunologic detection methods / Gary L. Bratthauer -- Theavidin-biotin complex (ABC) method and other avidin-biotinbinding methods / Gary L. Bratthauer -- Avidin-biotinlabeling of cellular antigens in cryostat-sectioned tissue/ Mark Raffeld and Elaine S. Jaffe -- Multiple antigenimmunostaining procedures / Tibor Krenacs, Laszlo Krenacs,and Mark Raffeld -- Immunoenzymatic quantitative analysisof antigens expressed on the cell surface (cell-ELISA) /Elaine Vicente Lourenço, Maria-Cristina Roque-Barreira --Use of immunogold with silver enhancement / ConstanceOliver -- Overview of flow cytometry and fluorescentprobes for flow cytometry / Robert E. Cunningham -- Tissuedisaggregation / Robert E. Cunningham -- Indirectimmunofluorescent labeling of viable cells / Robert E.Cunningham -- Indirect immunofluorescent labeling of fixedcells / Robert E. Cunningham -- Fluorescent labeling ofDNA / Robert E. Cunningham -- Deparaffinization andprocessing of pathologic material / Robert E. Cunningham -- Fixation and embedding / Constance Oliver and MariaCélia Jamur -- Preparation of colloidal gold / ConstanceOliver -- Conjugation of colloidal gold to proteins /Constance Oliver -- Colloidal gold/streptavidin methods /Constance Oliver -- Pre-embedding labeling methods /Constance Oliver -- Postembedding labeling methods /Constance Oliver -- The Clinical immunohistochemistrylaboratory : regulations and troubleshooting guidelines /Patricia A. Fetsch and Andrea Abati | | หัวเรื่อง |
Immunocytochemistry--Laboratory manuals | | หัวเรื่อง |
In situ hybridization--Laboratory manuals | | หัวเรื่อง |
Immunohistochemistry--methods--Laboratory Manuals | | หัวเรื่อง |
Immunohistochemistry--standards | | ผู้แต่งร่วม |
Jamur, Maria C& 33;lia | | ผู้แต่งร่วม |
Oliver, Constance |
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