| LEADER 00000cam 22004214a 4500 |
| 001 98 |
| 003 ULIBM |
| 008 120828s||||||||th 000 0 tha d |
| 020 ^a9781588294630 |
| 099 ^aIM7^bIm31I |
| 245 00 ^aImmunocytochemical methods and protocols/^bedited by Constance Oliver and Maria |
| 250 ^a3rd ed. |
| 260 ^aNew York, NY :^bHumana Press,^cc2010 |
| 300 ^axii, 416 p. :^bill. ;^c27 cm |
| 490 1 ^aMethods in molecular biology,^x1064-3745 ;^vv. 588 |
| 490 1 ^aSpringer protocols |
| 500 ^aPrevious ed.: 1999 |
| 504 ^aIncludes bibliographical references and index |
| 505 0 ^aOverview of antibodies for immunochemistry / ConstanceOliver and Maria Célia Jamur -- Introduction to thepurification of antibodies / Ana Cristina Grodzki and ElsaBerenstein -- Antibody purification : ammonium sulfatefractionation or gel filtration / Ana Cristina Grodzki andElsa Berenstein -- Antibody purification : ion-exchangechromatography / Ana Cristina Grodzki and Elsa Berenstein-- Antibody purification : affinity chromatography--Protein A and Protein G Sepharose / Ana Cristina Grodzkiand Elsa Berenstein -- Conjugation of fluorochromes toantibodies / Su-Yau Mao and J. Michael Mullins --Biotinylation of antibodies / Su-Yau Mao -- Cell fixativesfor immunostaining / Maria Célia Jamur and ConstanceOliver -- Permeabilization of cell membranes / Maria CéliaJamur and Constance Oliver -- Preparation of frozensections for analysis / Gary L. Bratthauer -- Processingof cytological specimens / Gary L. Bratthauer --Processing of tissue culture cells / Gary L. Bratthauer --Processing of tissue specimens / Gary L. Bratthauer --Heat induced antigen retrieval for immunohistochemicalreactions in routinely processed paraffin sections /Laszlo Krenacs, Tibor Krenacs, Eva Stelkovics, and MarkRaffeld -- Fluorochromes : properties and characteristics/ J. Michael Mullins -- Direct immunofluorescent labelingof cells / Maria Veronica Dávila Pástor -- Fluorescencelabeling of surface antigens of attached or suspendedtissue-culture cells / Mark C. Willingham -- Fluorescencelabeling of intracellular antigens of attached orsuspended tissue-culture cells / Mark C. Willingham --Fluorescent visualization of macromolecules in Drosophilawhole mounts / Ricardo Guelerman Pinheiro Ramos, LucianaClaudia Herculano Machado, and Livia Maria Rosatto Moda --Overview of conventional fluorescence photomicrography /J. Michael Mullins -- Overview of confocal microscopy /William D. Swaim -- Overview of laser microbeamapplications as related to antibody targeting / P. ScottPine -- Immuno-laser capture microdissection of rat brainneurons for real time quantitative PCR / Denis G. Baskinand L. Scot Bastian -- Overview of antigen detectionthrough enzymatic activity / Gary L. Bratthauer -- Theperoxidase-antiperoxidase (PAP) method and other all-immunologic detection methods / Gary L. Bratthauer -- Theavidin-biotin complex (ABC) method and other avidin-biotinbinding methods / Gary L. Bratthauer -- Avidin-biotinlabeling of cellular antigens in cryostat-sectioned tissue/ Mark Raffeld and Elaine S. Jaffe -- Multiple antigenimmunostaining procedures / Tibor Krenacs, Laszlo Krenacs,and Mark Raffeld -- Immunoenzymatic quantitative analysisof antigens expressed on the cell surface (cell-ELISA) /Elaine Vicente Lourenço, Maria-Cristina Roque-Barreira --Use of immunogold with silver enhancement / ConstanceOliver -- Overview of flow cytometry and fluorescentprobes for flow cytometry / Robert E. Cunningham -- Tissuedisaggregation / Robert E. Cunningham -- Indirectimmunofluorescent labeling of viable cells / Robert E.Cunningham -- Indirect immunofluorescent labeling of fixedcells / Robert E. Cunningham -- Fluorescent labeling ofDNA / Robert E. Cunningham -- Deparaffinization andprocessing of pathologic material / Robert E. Cunningham -- Fixation and embedding / Constance Oliver and MariaCélia Jamur -- Preparation of colloidal gold / ConstanceOliver -- Conjugation of colloidal gold to proteins /Constance Oliver -- Colloidal gold/streptavidin methods /Constance Oliver -- Pre-embedding labeling methods /Constance Oliver -- Postembedding labeling methods /Constance Oliver -- The Clinical immunohistochemistrylaboratory : regulations and troubleshooting guidelines /Patricia A. Fetsch and Andrea Abati |
| 650 0 ^aImmunocytochemistry^vLaboratory manuals |
| 650 0 ^aIn situ hybridization^vLaboratory manuals |
| 650 12 ^aImmunohistochemistry^xmethods^vLaboratory Manuals |
| 650 22 ^aImmunohistochemistry^xstandards |
| 655 2 ^aLaboratory Manuals |
| 700 1 ^aJamur, Maria C&^233;lia |
| 700 1 ^aOliver, Constance |
| 999 ^aอรทัย โคตรธาดา |
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